目的建立高效毛细管电泳法(HPCE)同时测定不同批次牛樟芝中腺嘌呤、腺苷、尿苷、鸟苷和肌苷等5种核苷类成分含量的方法。方法采用未涂渍标准熔融石英毛细管(75μm×64.5cm，有效长度56cm)为分离通道，60mmol·L-1硼砂(pH9.3)为运行缓冲液，分离电压为22kV，检测波长为260nm，毛细管温度为28℃，压力进样为50mbar×6s。结果5种核苷类成分的响应峰面积与其相应浓度的线性关系良好(r＞0.9995);加样回收率为98.83%～101.08%。实验表明，不同批次牛樟芝菌粉中5种核苷类成分的含量有所差异。结论该方法准确、可靠，重复性较好，可用于牛樟芝菌粉内在质量的评价和控制。
Abstract
OBJECTIVE To establish a high performance capillary electrophoresis (HPCE) method for simultaneous determination of five nucleosides and nucleobases, including adenine, adenosine, uridine, guanosine and inosine in antrodia camphorate of different batches. METHODS Based on the mode of HPCE, uncoated fused silica capillary (75 μm×64.5 cm , 56 cm of effective length) was used with separation voltage of 22 kV. 60 mmol·L-1 sodium borate was selected for the running buffer solution (pH 9.3). The detection wavelength was set at 260 nm. The sample was injected at 50 mbar×6 s and column temperature was maintained at 28 ℃. RESULTS The calibration curves of the five nucleosides showed good linearity (r>0.999 5). The average recoveries of the method were between 98.83%-101.08%. The contents of the five nucleosides in Antrodia camphorate samples of different batches were different. CONCLUSION The established method is reliable, accurate and can be used for the quality control of Antrodia camphorata.
关键词
高效毛细管电泳 /
牛樟芝 /
核苷 /
碱基 /
同时测定
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Key words
HPCE /
Antrodia camphorata /
nucleoside /
nucleobase /
simultaneous determination
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中图分类号：
R917
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参考文献
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脚注
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基金
江苏省中药炮制重点实验室开放式课题(ZYPZ007);江苏省高校优秀科技创新团队“中药资源化学研究”(2011);江苏高校优势学科建设工程资助项目(ysxk-2010)
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